| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lisnow, Dylan (17)
|
MALCY |
51.14 |
51.05 |
-0.09 |
| 2 |
Urbine, Wyatt V (17)
|
MAJY |
50.24 |
51.43 |
1.19 |
| 3 |
Zhu, Eric Y (17)
|
MABRY |
50.35 |
51.86 |
1.51 |
| 4 |
Byrd, Bryan (17)
|
MAWEST |
49.48 |
52.01 |
2.53 |
| 5 |
Inman, Jack C (16)
|
MALCY |
51.74 |
52.55 |
0.81 |
| 6 |
Slupe, Collin D (16)
|
MAKAY |
51.24 |
52.68 |
1.44 |
| 7 |
McLaughlin, Thomas D (16)
|
MARAYS |
51.50 |
52.87 |
1.37 |
| 8 |
Braithwaite, Dominic J (16)
|
MAKAY |
55.90 |
53.36 |
-2.54 |
| 9 |
Zwilgmeyer, Matthew (18)
|
MAWEST |
50.25 |
53.82 |
3.57 |
| 10 |
Peterson, JB (16)
|
MARAYS |
55.63 |
53.99 |
-1.64 |
| 11 |
Iacono, Maximus F (15)
|
MABRY |
55.17 |
54.00 |
-1.17 |
| 12 |
Myers, Connor K (18)
|
MALCY |
54.47 |
54.22 |
-0.25 |
| 13 |
Ronayne, Thomas J (15)
|
MARAYS |
53.82 |
54.73 |
0.91 |
| 14 |
Spruance, Jacob V (16)
|
MAKAY |
57.44 |
54.87 |
-2.57 |
| 15 |
Jiang, Ishau L (15)
|
MAUMLY |
58.98 |
55.28 |
-3.70 |
| 16 |
Yu, David S (17)
|
MAUMLY |
54.50 |
55.53 |
1.03 |
| 17 |
McGroary, Sean P (16)
|
MARAYS |
57.17 |
55.55 |
-1.62 |
| 18 |
Tuffner, Cole J (15)
|
MALCY |
57.73 |
55.79 |
-1.94 |
| 19 |
Light, Gunnar I (15)
|
MABRY |
57.71 |
56.69 |
-1.02 |
| 20 |
Borda, Danny B (16)
|
MALCY |
56.31 |
57.35 |
1.04 |
| 21 |
Weidner, Christopher J (17)
|
MAWEST |
1:00.44 |
57.48 |
-2.96 |
| 22 |
Londono, Santiago (16)
|
MAWEST |
1:01.37 |
1:00.27 |
-1.10 |
| 23 |
Oh, James H (15)
|
MAUMLY |
58.61 |
1:02.03 |
3.42 |
| 24 |
Taylor, Jack H (15)
|
MAWEST |
1:07.45 |
1:10.91 |
3.46 |
| 25 |
Anne, Akash (15)
|
MAUMLY |
|
1:16.20 |
- |
|
McGann, Frankie P (15)
|
MARAYS |
55.55 |
NS |
- |
|
Jones, Aaron M (17)
|
MAJY |
53.67 |
NS |
- |
|
Weidner, Gregory S (15)
|
MAWEST |
56.11 |
NS |
- |